Penaeus vannamei broodstock genetic diversity based on ۱۶S rRNA gene mtDNA

Based on the ۱۶S rRNA genome of mitochondrial DNA, this study examined the genetic diversity of Penaeus vannamei broodstocks to determine genetic indicators. This study included two populations, High Health (H) and Molokai (M), of the F۳ and F۵ generations, respectively. Extraction of DNA from samples was performed using a commercial kit. A pair of special primers amplified the ۱۶S rRNA gene. Then, a ۱۶S rRNA fragment of mitochondrial DNA was sequenced. The World Gene Bank has accession numbers LC۴۶۸۱۲۹.۱ and LC۴۶۸۱۳۰.۱ for these sequences. Based on the ۴۸۶ loci identified, there are ۴۸۴ conserved loci and two variable loci, each of which contains phylogenetic information under parsimony conditions, and ۱۸ singletons. The similarity between LC۴۶۸۱۲۹.۱ and LC۴۶۸۱۳۰.۱ with LC۱۲۱۷۰۱.۱ was ۹۹.۴۹ and ۹۸.۹۹%, respectively. In conclusion, Neighbor-joining algorithms identified each population with accession number LC۴۶۸۱۲۹.۱ and LC۴۶۸۱۳۰.۱ with ۶۴% and ۶۶% in separate branches, respectively.

کلیدواژه ها:

Penaeus vannamei ، ۱۶S rRNA ، Genetic indicators ، Phylogenetic