Research Article: A comparative study on the nutritional and cytotoxic potentials of the polysaccharides extracted from Chlorella vulgaris

Chlorella, a green microalga, is valued in food and medicine for its high nutritional and functional properties. Reactive oxygen species can cause oxidation reactions linked to cardiovascular diseases, degenerative conditions, and cancer. However, bioactive compounds and carotenoids in microalgae can help to alleviate certain disease symptoms by protecting tissues. Polysaccharides from C. vulgaris were extracted using a hot water method and their antioxidant activity was evaluated through the DPPH radical scavenging method and reducing power. The impact on the death rate of mouse colon cancer cells (CT-۲۶) was also assessed using the ۲, ۵-diphenyl-۲H-tetrazolium bromide (MTT) method. The analysis showed that C. vulgaris contained ۱۹.۰۸±۰.۰۹ w/v % polysaccharides and yielded about ۵% of its dry weight. The extracted polysaccharides demonstrated antioxidant activity with the highest DPPH inhibition and reducing power, measuring ۹۱.۱۵% and ۰.۷۸۱% at ۶ mg/mL, respectively. BHA at ۰.۰۳۱ mg/mL exhibited DPPH scavenging (۳۹.۷۸%) and reduced the power (۳.۶۵%). The polysaccharides also showed cytotoxic effects, specifically against CT-۲۶ cells with an IC۵۰ value of ۴۸۱.۵۳μg/mL after ۴۸ h. These findings suggest that polysaccharides from C. vulgaris may possess potential cytotoxic properties against tumor cell lines.

کلیدواژه ها:

Cytotoxicity ، Polysaccharides ، Chlorella vulgaris ، Biochemical compounds