Stem Cells in Aesthetic Dermatology: From Wound Healing to Skin Rejuvenation through PEG۱۰-Mediated Regulation

Mesenchymal stem cells (MSCs), particularly adipose-derived MSCs (ADMSCs), have gained attention in aesthetic dermatology due to their regenerative properties and ability to restore dermal structure and function. However, the molecular mechanisms driving their effects remain incompletely understood. This study investigates the role of the imprinted gene PEG۱۰ in enhancing skin rejuvenation and wound healing via transcriptional, post-transcriptional, and signaling mechanisms. PEG۱۰ was overexpressed or silenced in human ADMSCs, and its effects on wound healing and skin rejuvenation were evaluated using scratch assays and a ۳D skin equivalent model. RNA-seq and microarray data were analyzed to identify differentially expressed genes (DEGs). Bioinformatic analyses included Gene Ontology (GO), KEGG/Reactome pathway enrichment, protein–protein interaction (PPI) networks, and microRNA target prediction. PEG۱۰ overexpression enhanced cell migration, collagen I/III production, and elastin expression in vitro. Transcriptomic profiling revealed upregulation of ECM-related genes (COL۱A۱, MMP۹, FGF۲) and enrichment of TGF-β, Wnt/β-catenin, and PI۳K-Akt pathways. PPI networks identified key regenerative hubs, while miRNA analysis implicated miR-۱۴۱ and miR-۲۰۰c in post-transcriptional PEG۱۰ regulation. PEG۱۰ plays a pivotal role in regulating ADMSC-mediated skin regeneration through integrated gene networks and signaling pathways. These findings identify PEG۱۰ as a promising molecular target for therapeutic strategies in aesthetic and regenerative dermatology.

کلیدواژه ها:

PEG۱۰ ، Mesenchymal stem cells ، Skin rejuvenation ، Wound healing ، Aesthetic dermatology