Flow Cytometric Analysis of Programmed Cell Death in Rose (Rosa hybrida cv. Dolce vita+) as Influenced by Physicochemical Treatments

This study aimed to increase the vase life of cut rose flowers by improving the regulation of Programmed Cell Death (PCD). Experiments were carried out on cut rose (Rosa hybrida cv. Dolce vita+) flowers under either physical treatment of Static Magnetic Field (SMF: ۱۵ and ۲۵ mT) for ۳ hours, or chemical treatments of silver nano particle (Nano-Ag: ۵ and ۱۰ ppm), ۶-Benzyladenine (BA: ۲۵ and ۵۰ mg L-۱), ۱% sucrose, and combinations of ۵ and ۱۰ ppm nano-Ag with ۳ and ۶% sucrose. Results showed that a ۱۵ mT-SMF significantly increased vase life up to ۲۵ days, compared to the controls and to all chemical treatments. Among the chemicals, ۵ ppm Nano-Ag and ۱% (w/v) sucrose increased vase life to ۲۳ and ۱۸ days, respectively. The smallest decline in fresh weight was observed in the ۱۵ mT-SMF physical treatment. Markedly, the ۱۵ mT-SMF treatment led to the least reduction in Chlorophyll (Chl) content. On the ۱۷th day of the applied different treatments, both Water Uptake (WU) and Relative Fresh Weight (RFW) showed an inverse significant relationship with PCD in cut rose flowers, verifying the delayed PCD, which is favored in the market. As a whole, the most effective induced treatments (۱۵ mT-SMF, ۵ ppm nano-Ag, and ۱% sucrose) are suggested to be promising for enhancing postharvest quality and prolonged vase life of cut rose flowers.

کلیدواژه ها:

Silver nanoparticle (Nano-Ag) ، ۶-Benzyladenine (BA) ، Static Magnetic Field (SMF) ، Vase life.