Development And Characterization Of An Optimized Alginate/Gelatin/ORC/ZnO Nanocomposite For Hemostatic And Antibacterial Applications

Excessive bleeding remains a major cause of mortality in trauma, surgery, and emergency situations, highlighting the urgent need for effective hemostatic materials. In this study, a novel composite hemostatic agent was developed by combining sodium alginate, gelatin, oxidized regenerated cellulose (ORC), and zinc oxide (ZnO) nanoparticles. The composite was optimized with a ۷۵:۲۵ alginate-to-gelatin ratio, ۱% ORC, and ۳% ZnO nanoparticles. Comprehensive characterization was performed using FTIR, XRD, water absorption, swelling ratio, carboxyl group quantification, and antibacterial assays. The optimized composite exhibited superior water absorption and swelling capacity, along with a high content of carboxyl groups, which are critical for coagulation. Antibacterial testing according to the AATCC ۱۰۰ standard revealed significant inhibition of both E. coli and S. aureus, attributed to the synergistic effects of ZnO nanoparticles and the biopolymer matrix. The enhanced physicochemical and biological properties of the composite suggest its strong potential as an advanced hemostatic material for wound care and surgical applications. This study demonstrates that the integration of biocompatible polymers and functional nanomaterials can lead to the development of multifunctional wound dressings with both hemostatic and antibacterial efficacy.

کلیدواژه ها:

Hemostatic composite ، Alginate ، Gelatin ، Oxidized regenerated cellulose ، Zinc oxide nanoparticles