Protective effect of Henna (Lawsonia inermis L.) fixed oil (a Persian medicine preparation) on acetic acid-induced ulcerative colitis in rats

Objective: Ulcerative colitis is a chronic recurrent inflammatory bowel disease of unknown etiology. The anti-inflammatory, immunomodulatory, and antioxidant characteristics of Henna (Lawsonia inermis) fixed oil (HFO) imply that it may be advantageous for the treatment of colitis.Materials and Methods: In this research, the effect of HFO in a Wistar albino rat model of acetic acid (AA)-induced ulcerative colitis, was examined. The animals received daily oral administration of either normal saline (۱۰ ml/kg), HFO (۱۰۰, ۴۰۰, and ۱۶۰۰ µl/kg), or dexamethasone (۲ mg/kg) for ۵ days. A single intracolonic injection of ۲ ml of a ۴% (v/v) acetic acid solution was used to induce colitis. The levels of myeloperoxidase (MPO) and tumor necrosis factor-alpha (TNF-α) were measured.Results: The administration of HFO at doses ۴۰۰ and ۱۶۰۰ μl/kg showed a significant enhancement in the weight-to-length ratio of colon tissue in comparison to the control group. Furthermore, the increased amounts of HFO (۴۰۰ and ۱۶۰۰ μl/kg) were associated with a significant reduction in ulcer severity, area, and index. However, examination of tissue samples revealed a decrease in the overall colitis index suggesting fewer inflammatory cells invaded the colonic regions of rats treated with HFO at doses of ۴۰۰ and ۱۶۰۰ μl/kg. Moreover, the elevated MPO levels and TNF-α were significantly decreased following the administration of the fixed oil at these doses.Conclusion: These findings indicate that HFO could potentially decrease the manifestations of experimental colitis in a dose-dependent manner.

کلیدواژه ها:

Ulcerative colitis ، Lawsonia inermis ، Fixed oil ، Inflammation ، Rat