Lipoxygenase-۱ Leaching from Soybean Flour Employing Stirred Tank Contactor

The present work examines leaching of lipoxygenase-۱ from soybean flour employing stirred tank vessel. The effect of operating parameters, impellers speed, operational period, temperature, pH and scale-up were considered.The acetic acid pH: ۵.۲ increased the leaching of lipoxygenase- ۱.The sensitivity of agitator speed and geometrical-scale-up on the enzymeleaching has been conducted. The effect of agitator speed on the geometrically- scaled- up reactor has shown that similar amount of enzyme is leached at lower speed. The result is in para with the conventional system. The importance of operational period from ۵ to ۵۰ min on the enzyme leaching was evaluated and higher enzyme leaching obtained within ۱۰ min. of operation. The Sigmaplot-۶ for statistical verification and developing a correlation for the enzyme leaching was used.

کلیدواژه ها:

Enzyme leaching ، Lipooxygenase-۱ ، Operational period ، Soybean flour ، Stirred tank contactor