Investigation of Antibacterial and Cytotoxicity Effect of Green Synthesized TiO۲ Nanocomposites, an Experimental and Theoretical Study

Protecting the hair, skin, or products of itself are utilized by sunscreen filters which were frequently blocked hazardous UV-Vis radiation. Considering its photoprotective impact on the skin facing the radiation of ultraviolet and visible, TiO۲ is a common and cost-efficient photocatalytic structure utilized in sunscreens. In this research, the continual process was done to optimize the green synthesis of TiO۲ nanoparticles and nanocomposites through a new, easy, cost-efficient, and quick approach to making nanostructures utilizing a sonochemistry method. SiO۲, Al۲O۳, ZnO, and MnO were utilized to compose green synthesized TiO۲ nanoparticles for this purpose. The samples were recognized by XRD, FT-IR, DLS, and SEM. Also, the cytotoxicity and antibacterial activity were assessed. DFT computation was performed to identify the connected energy and band gap energy of nanocomposites by B۳LYP/Lan۲DZ quantum approach. TiO۲/Al۲O۳ showed a lower size and the lowest agglomeration than synthesized TiO۲ and other nanocomposites.  Furthermore, all samples indicated strong antibacterial activity against investigated bacteria due to cell death caused by membrane permeability increase and bacterial wall integrity disruption. Nanostructures have cytotoxicity with a low level on A۱۷۲ cells. The only exception is TiO۲/ZnO which indicated a potent index of cytotoxicity on the cancerous cell lines as demonstrated by a low IC۵۰ value of ۵۰ ppm.  The relative energy and band gap of nanocomposites indicated that TiO۲/Al۲O۳ has the best stability in chemical and biochemical mediums among other nanocomposites.  These green synthesized TiO۲/Al۲O۳ nanostructures may have promising applications in nanoformulation to combat bacterial infections in the future.

کلیدواژه ها:

Titanium dioxide ، Materials chemistry ، Environmental friendly synthesis ، TiO۲ nanocomposites ، Antibacterial ، DFT calculations