Investigation of Physicochemical, Antioxidant, and Antimicrobial Properties of Thymol and Resveratrol Encapsulated with Zein-Caseinate Nanoparticle

Thymol and resveratrol are organic, phenolic compounds with wide-ranging potential applications in the food industry, mainly use as an anti-oxidation and antibacterial additives. However, their weak stability, low water solubility, and bioavailability have severely limited their industrial applications. Herein, zein-casein nanoparticles loaded with thymol and resveratrol have been successfully prepared using the liquid-liquid dispersion method to empower their stability, solubility, antioxidant, and antimicrobial properties. As a result, formulation of ۷۵% thymol with ۲۵% resveratrol mixing ratio ۱:۲۰ (۷۵T۲۵R۲۰) was the most stable nanoparticle with zeta potential of -۱۹.۵۴ compared to only thymol or resveratrol nanocapsules (۱۰۰T۰R۲۰ and ۰T۱۰۰R۲۰) with zeta potential of -۱.۲۲ and -۴.۱۲ respectively.  In addition, scavenging of ۲,۲-diphenyl-۱-picrylhydrazyl (DPPH) radical activity increased to ۵۸.۷۷% in ۷۵T۲۵R۱۰ nanocapsule compared to the antioxidant activity of nanocapsule of only thymol or resveratrol with DPPH of ۲۰.۳۳% and ۱۳.۶۷% respectively. The synergic effect of the thymol and resveratrol also improved the antimicrobial activity. For example, Minimum Inhibitory Concentration (MIC) against e.coli bacteria in culture medium, drops from ۳۰۰-۳۵۰ for sole thymol/resveratrol nanocampule (۱۰۰T۰R۱۰, ۰T۱۰۰R۱۰) to ۱۰۰ for ۷۵T۲۵R۱۰. Finally, Scanning Electron Microscopy (SEM), and Fourier Transforms InfraRed (FT-IR) spectroscopy have been used for the physical characterization of the resultant NanoParticles (NPs). It is worth mentioning that formed zein-casein nanoparticles loaded with thymol and resveratrol have good redispersibility and physical stability stored at -۲۰ ۰C. Therefore, this investigation introduces an excellent organic candidate for delivering hydrophobic active compounds, which is of significance in the cosmetics and especially food industries.

کلیدواژه ها:

liquid-liquid dispersion method ، Antioxidant ، caseinate ، nanoparticles ، Phenolic compounds