Extraction of Borneol and Bornyl Acetete from Ferulago Macrocarpa (Fenzl) Boiss. Using Hydrodistillation-Headspace Solvent Microextraction and Chemometrics Approach

In this study, the ability of concurrent headspace solvent micro-extraction coupled with continuous hydro-distillation (HD-HSME) as an extraction and pre-concentration technique is presented for the analysis of Ferulago macrocarpa essential oil before GC analysis. A chemometric approach has allowed to enhancement of the extraction efficiency of two major terpenoids of Ferulago macrocarpa essential oil, namely borneol and bornyl acetate, with a limited number of experiments according to the Box-Behenken design in a random order. The considered variables in this technique were sample mass, micro-drop volume of n-hexadecane, and extraction time. The results showed that the chromatographic peak areas of two major terpenoids of Ferulago macrocarpa, namely borneol and bornyl acetate, regularly change as a function of three variables (R۲ ≥ ۰.۹۹). Furthermore, the box-behenken design successfully permitted the study of the main and interactive effects of variables, which described this dependence using the MLR model.  Finally, the optimum conditions for micro-extraction of borneol and bornyl acetate from Ferulago macrocarpa essential oil included ۳ and ۴.۲ g of dried-powdered leaves, ۵ and ۳ µL of micro-drop volume, and the extraction time of ۷ minutes, respectively.

کلیدواژه ها:

Hydrodistillation-headspace solvent microextraction ، Ferulago macrocarpa essential oil ، Borneol ، bornyl acetate ، Multivariate analysis ، Box-Behenken design