Parsley Exosomes for Wound Healing Application

Exosomes are extracellular nanovesicles that resemble plasma membranes and are released by various cells to facilitate intercellular communication and modulate immune responses against pathogens. Their internal contents are safeguarded by a bilayer membrane. Plant-derived exosomes have distinct characteristics, and given the challenges and costs associated with extracting human exosomes—along with their potential to activate the immune system—plant exosomes may be a more advantageous alternative. This study is the first to extract and characterize exosomes from parsley, focusing on their morphological features and effects on healthy human fibroblast cells. The ultracentrifugation method was employed to isolate the exosomes, which were then prepared for dynamic light scattering (DLS) and scanning electron microscopy (SEM) analysis. The findings indicated that parsley exosomes are quite small, measuring approximately ۷۰ nm, with a negative zeta potential. Subsequent tests, including the Bradford assay, were conducted to assess the presence of proteins within the exosomes, revealing a significant protein content. Additionally, polysaccharide compounds and freeze-drying techniques were utilized to stabilize the exosomes. Ultimately, the positive effects on fibroblast cell proliferation suggest that these exosomes hold great promise for wound healing applications.

کلیدواژه ها:

exosome ، wound healing ، parsley