An Electrospun Nanofiber with Vessel-derived Extracellular Matrix for Wound Healing Application

The design of tissue engineering composites has introduced a promising strategy for skin substitutes and overcoming the limitations of autologous grafts. The ideal skin substitute should be permeable, biocompatible, and biodegradable while creating a physical barrier against damage and the entry of microorganisms. In this study, we aimed to construct and investigate three-dimensional biological scaffolds based on aortic Extracellular matrix (ECM) and hybrid polymeric nanofibers for repairing full-thickness wounds. Therefore, we developed hybrid polymeric nanofibers, followed by incorporating vessel-derived ECM into the composition. Having created the construct, its associated effect on the structure of hybrid polymeric nanofibers was investigated, and our findings showed that the electrospun nanofibers showed favorable mechanical performance and cell compatibility. In addition, dynamic cell cultures of human fibroblast cells (HDF) on composite were given to be interpreted by a professional expert in this area. A cell culture study showed that the proliferation of HDF cells was enhanced, and the cell attachment and viability were improved significantly. These findings suggested that scaffolds can be an appropriate choice for wound healing.

کلیدواژه ها:

Wound healing ، Electrospinning ، Extracellular Matrix ، Hybrid