Antibody Engineering Toward Enhancement of Teplizumab Anti-CD۳ Binding Affinity in Type ۱ Diabetes Prevention and Treatment

Objective: The monoclonal antibodies with CD۳ target have the potential to change the progression of type ۱ diabetes (T۱D) and enhance the longevity of beta-cell function. The main objective of the study is antibody engineering toward Enhancement of Teplizumab Anti-CD۳ Binding Affinity in T۱D prevention and treatment. Materials and Methods: We aimed at finding the important amino acids of this antibody, and then replaced these amino acids with others to improve their binding affinity, and examine the binding affinity of antibody variants to antigens. In the end, we selected high-affinity variants of the antibody according to results of High Ambiguity Driven biomolecular DOCKing (HADDOCK). Results: Our research indicated that ۱۴ mutated variants were able to enhance the binding characteristics of Ab in comparison to standard antibodies. Conclusion: The altered antibodies could serve as promising options for enhanced affinity binding to antigens, which could affect the specificity and sensitivity of antibodies.

کلیدواژه ها:

Teplizumab ، Antibody Engineering ، Type ۱ diabetes