Isolation and Screening of Antibacterial-Producing Endophytic Actinomycetes from Hollyhock (Alcea rosea)

Actinomycete bacteria are widely recognized for their ability to produce a diverse array of bioactive compounds, particularly antibiotics, and contribute significantly to the discovery of naturally derived antimicrobial agents. This study focused on the isolation and characterization of actinomycete strains from hollyhock (Alcea rosea) plant tissues to assess their antibacterial potential against clinically relevant pathogens. The target pathogens included Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. A total of ۱۱۰ bacterial isolates were obtained, with ۶۲ isolates confirmed as Actinomycetes through PCR amplification of the ۱۶S rRNA gene. Approximately ۴۶.۵% of these isolates exhibited antibacterial activity, with pronounced effects against B. cereus and S. aureus, suggesting their potential role in combating antibiotic-resistant bacteria. Genetic analysis revealed the presence of non-ribosomal peptide synthetase (NRPS) genes, indicating that the produced antimicrobial compounds may originate from NRPS pathways. However, no polyketide synthase (PKS) genes were detected, which may limit the diversity of bioactive metabolites. These findings highlight the pharmaceutical potential of hollyhock-associated Actinomycetes as promising sources of novel antimicrobial agents.

کلیدواژه ها:

Bioprospecting ، Antibacterial Properties ، Actinomycetes ، Endophytes ، Alcea rosea