Investigating the effect of the melatonin hormone on growth indicators and biochemical compounds of Chlorella vulgaris microalgae

Melatonin is one of the growth hormones that can induce growth and primary and secondary metabolites in microalgae. Green microalgae, including Chlorella vulgaris can be considered renewable sources of biomass and secondary metabolites. This research was studied to investigate the effects of this hormone on the growth and biochemical parameters of Chlorella vulgaris microalgae. For this purpose, microalgae were cultured under concentrations of ۰, ۰.۱, ۱, ۱۰, ۱۰۰ and ۵۰۰ µM melatonin. After ۲۱ days, microalgae were used to measure parameters. The results showed that the maximum productivity of biomass, cell number, and chlorophyll pigments were observed under the concentration of ۱۰ µM and the lowest value of these parameters was observed at the concentration of ۵۰۰ µM melatonin. Also, the maximum amount astaxanthin and lutein pigments, phenolic compounds and antioxidant capacity were obtained under the concentration of ۵۰۰ μM. According to the above data, it can be suggested that melatonin, by producing reactive oxygen species and its regulatory role, induces growth parameters and chlorophyll pigments in optimal concentrations, and on the other hand, by increasing PAL enzyme activity, the content of phenolic compounds and increases carotenoid pigments. The increase in these compounds ultimately leads to the improvement of the antioxidant capacity of microalgae.

کلیدواژه ها:

Biomass ، Carotenoids ، Phenolic compounds ، Melatonin ، ترکیبات فنلی ، زیست توده ، کاروتنوئید ، ملاتونین