Green Synthesis of Gold Nanoparticles Using Cannabis Seed Extract and Evaluation of Their Biological Properties

Gold nanoparticles, valued for their non-toxicity, are crucial in medicine, food packaging, and electronics. This study highlights the green synthesis of gold nanoparticles using Cannabis extract and examines their biological properties. Cannabis aqueous extract was prepared via maceration, and nanoparticles were synthesized with ۲mM HAuCl۴, indicated by a yellow-to-purple color change. Ultraviolet-visible (UV-Vis) spectroscopy (λmax=۵۳۰nm) confirmed synthesis, while Field Emission Scanning Electron Microscopy (FESEM) and X-ray Diffraction (XRD) analyzed morphology and crystallography. Proteins were detected using the Biuret reagent, and antioxidant properties were evaluated via the DPPH method. XRD patterns aligned with standard gold nanoparticles. The nanoparticles exhibited good aggregation and varied shapes, including spherical, polygonal, and triangular, with promising antioxidant activity (IC۵۰=۶۴.۳۵mg/L).

کلیدواژه ها:

Cannabis seed ، Antioxidant ، Protein ، Gold Nanoparticles ، Green Synthesis