Optimizing Porosity and Pore Size of Nanofibrous Scaffolds for Enhanced Neural Cell Growth and Proliferation

Nanofibrous scaffolds are essential in tissue engineering, closely resembling the extracellular matrix to support cell adhesion and growth. In this study, polycaprolactone (PCL) nanofibrous layers with varying porosities and pore sizes were fabricated via electrospinning to assess their effects on A-۱۷۲ neural cell behavior. The scaffolds were produced on collectors with distinct mesh sizes, resulting in structural differences. Among the scaffolds, the one fabricated on a collector with ۵۸% mesh porosity (type ۴) demonstrated superior performance, with a ۴۴% improvement in cell proliferation compared to flat collectors. This scaffold’s optimized porosity (۹۶%) and moderate pore size (۰.۴۲–۲۳ µm) created favorable conditions for cell adhesion, proliferation, and nutrient transport. These findings highlight the critical role of scaffold architecture in enhancing neural cell behavior and advancing tissue regeneration strategies.

کلیدواژه ها:

Nanofibrous scaffold ، Neural cells ، Porosity ، Electrospinning ، Tissue engineering