Enzybiotics, enzymes with antimicrobial activities, have emerged as promising alternatives to traditional antibiotics because of their high specificity and efficacy against resistant bacteria. This study emphasizes the pivotal role of marine bacteria in the production of enzybiotics and addresses the urgent need for novel antimicrobial agents to combat antibiotic resistance. Marine ecosystems, particularly those with extreme conditions, harbor bacteria that have evolved robust enzybiotic production mechanisms along with potent resistance strategies. This study investigated metagenomic datasets from polluted and non-polluted environments across six distinct habitats using computational approaches to explore the co-abundance patterns of enzybiotic-producing bacteria. BLAST analyses with NCBI-BLAST+ identified sequences using ۱۰۰% identity, and organismal frequency was used as an indicator of co-abundance. Correlations between bacterial species were calculated using the Himsc package, and a co-abundance network was visualized in Cytoscape, revealing critical interactions among key marine bacteria such as Marinomonas mediterranea, Pseudoalteromonas tunicata, and Pseudoalteromonas luteoviolacea. The findings showed a strong positive correlation between M. mediterranea and P. tunicata (correlation coefficient = ۰.۹۹۰۲, p = ۰.۰۰۰۱), suggesting cooperative interactions driven by shared enzybiotic activities, including antifouling compound production and oxidative enzymes, such as laccases and tyrosinases. These enzymes are crucial for biofilm formation, cell death regulation, and ecological synergy, thereby enhancing their coexistence and functional output in marine ecosystems. In contrast, significant negative correlations, such as between P. tunicata and P. luteoviolacea (correlation coefficient = -۰.۹۲۶۶, p = ۰.۰۰۷۹), highlighting competitive exclusion mechanisms. The inhibitory effects of broad-spectrum enzybiotics produced by P. tunicata likely suppress the growth of competing species, while M. mediterranea employs marinocine, a hydrogen peroxide-generating protein, to inhibit competitors like P. luteoviolacea thereby gaining a competitive advantage. These results underscore the dual roles of enzybiotic activity in fostering cooperative interactions and driving competitive exclusion within microbial communities. Such dynamics not only shape microbial ecosystem structures but also reveal potential applications for harnessing enzybiotics in combating antibiotic resistance. Cooperative activities, as seen between M. mediterranea and P. tunicata, demonstrated the potential of enzybiotics to enhance ecological functionality, while competitive dynamics provided insights into microbial strategies for dominance in shared environments. Further experimental validation is essential to confirm
