Cartilage-Specific Protein Expression in Adipose-Derived Stem Cells Treated with Pomegranate Seed Extract and Soybean/Avocado in Fibrin Scaffolds

Introduction: Due to the lack of repair of cartilage lesions, tissue engineering tries to produce cartilage using cells, scaffolds, and growth factors. Pomegranate seed extract (PSE) and unsaponifiable soybean/ avocado (ASU) are plant compounds that effectively maintain the extracellular cartilage matrix. In this study, we tried to investigate the effects of PSE on the process of chondrogenesis and compared it with the effects of ASU in this process. Materials and Methods: Human adipose-derived stem cells (hADSCs) were transferred to fibrin scaffolds in the third passage in three groups (control, PSE, and ASU) for chondrogenic differentiation. After ۱۴ days, Western blotting evaluated the samples for cell survival by (۳-[۴,۵-dimethylthiazol-۲-yl]-۲,۵ diphenyl tetrazolium bromide) (MTT) assay production of collagen type II and collagen type X. Results: Evaluation of MTT results in different groups showed that the survival rate in the two groups of PSE and ASU was significantly lower than the control group (p ≤ ۰.۰۵). Quantitative western blotting showed that the production of collagen type II protein in both groups significantly increased compared to the control group (p ≤ ۰.۰۵). Quantitative analysis of collagen type X protein production showed that the production of this protein in the PSE group was significantly reduced compared to the ASU group. Conclusion: PSE and ASU are two important factors in inducing chondrogenesis in hADSCs in fibrin scaffold. ASU's impact on chondrogenesis of hADSCs in fibrin scaffold is greater than that of PSE.

کلیدواژه ها:

Avocado/Soy ، Chondrogenesis ، Collagen ، Pomegranate Seed Extract ، Protein ، پروتئین ، کلاژن ، عصاره هسته انار ، آووکادو/سویا ، کندروژنز