Investigating the Phenophysiological Traits of Transgenic Rapeseed Lines (Brassica napus) with aroA Gene Harboring a Point Mutation of Proline ۱۰۱ to Serine (P۱۰۱S) under Glyphosate Herbicide Treatment

One of the most effective ways to breed glyphosate-resistant plants is to reduce the affinity of glyphosate to the EPSPS enzyme by manipulating the aroA gene. In this study, P۱۰۱S mutation was induced in the aroA of E. coli and the mutant gene was cloned in the pUC۱۸ plasmid. It was transferred by Agrobacterium tumefaciens to the rapeseed. To investigate the phenophysiological traits in transgenic rapeseed lines under different glyphosate treatments (۰, ۱.۲, ۲.۴, ۴.۸, ۹.۶, ۱۹.۲, ۳۸.۴, ۷۶.۸ and ۱۵۳.۶ mM), the seeds of T۲ generation transgenic plants were studied in greenhouse conditions in a factorial experiment. The results showed that the control (no herbicide) had the lowest amount of days to flowering (۳۲.۵ days), partial water pressure (۱۴.۹۲kPa), active photosynthetic radiation (۳۹۳.۴ mmol m−۲ s−۱), and leaf surface temperature (۲۸.۸۸۰°C). The concentration of ۲.۴ mM had the highest stomatal conductance (۰.۵۷۳ mol m−۲ s−۱) and photosynthesis rate (۱۴.۰۷ μmol m−۲ s−۱). The lowest value of stomatal resistance was related to the concentration of ۴.۸ mM (۲۴۶.۹ mmol m−۲ s−۱), and the lowest value of stomatal conductance was associated with ۹.۶ mM (۰.۰۴۷ mol m−۲ s−۱). The highest rate of CO۲ source (۴۱۶.۳ ppm) and active photosynthetic radiation (۵۷۶.۹ mmol m−۲ s−۱) was seen in the ۱۹.۲ mM. The ۳۸.۴ mM had the highest number of days to flowering (۴۸.۱ days) and leaf surface temperature (۳۸.۷۴۸°C) and the lowest amount of CO۲ source (۳۸۶ppm). The ۷۶.۸ mM had the highest stomatal resistance (۳۳۰.۲ mmol m−۲ s−۱) and the lowest photosynthesis rate (۱.۳۶ μmol m−۲ s−۱). The highest partial pressure of the water source was related to ۱۵۳.۶ mM (۱۹.۰۷ kPa). In summary, different concentrations of herbicides exhibit varying degrees of phenophysiological traits, and desired traits can be improved based on these concentrations.

کلیدواژه ها:

EPSPS ، Glyphosate ، Herbicide Resistance ، Physiological traits ، Rapeseed