Evaluation the effect of sea cucumber (Holothuria leucospilota) on invA gene expression in Salmonella infantis

Introduction: The invA gene plays a critical role in the pathogenicity of Salmonella infantis. Marine organisms, including sea cucumbers due to their effective secondary metabolites, have been identified and studied with compounds with antioxidant, anti-cancer, antibacterial and anti-inflammatory properties. The aim of this study is to determine effect of sea cucumber extraction (Holothuria leucospilota)  on S. infantis invA gene expression . Material & Methods: Poultry meat was sampled. The S. infantis strains containing invA gene were isolated. The Hexane extract was extracted from sea cucumber colon tissue. Its effects on S. infantis and its effect on gene expression were investigated by MIC and Real-time PCR, respectively. Results: Morphological and biochemical characteristics of these bacteria were confirmed. From ۴۵۰ samples, ۱۲ S.infantis isolates were isolated. The PCR technique was used to identify the invA encoding gene. All ۱۲ isolates have invA virulence genes. MIC was determined ۲۵۶ µg/ml. The effect of sea cucumber extract on invA gene expression in S.infantis was evaluated, and the rate of change for the invA gene is estimated -۱.۲۱. Discussion & Conclusion: According to our results, hexane extract extracted from the sea cucumber (H. leucospilota) caused reduction ofinvA gene expression in salmonella infantis. So, it can be used as a therapeutic supplement against S. infantis

کلیدواژه ها:

Salmonella infantis ، Extracts of the sea cucumber ، Antimicrobial activity ، Gene expression ، سالمونلا اینفنتیس ، خیار دریایی ، فعالیت ضدباکتریایی ، بیان ژن