Evaluation of Human Papilloma Virus (HPV) genotypes diversity in oral cancer lesions of patients in Imam Khomeini Hospital

Introduction: Squamous cell carcinoma comprises approximately ۹۴% of all oral cavities. One reasons for this cancer is Human Papilloma Virus (HPV) with different genotypes .Finding the most common genotypes will be helpful to control and prevent of spreading this cancer.Materials and Method: ۷۰ Paraffinated blocks were collected from cancer department of Imam Khomeini hospital in Tehran. All of these samples included histopathological report of dysplastic lesions .They weredeparaffinated.۴ primers were designed for PCR .Then they were transferred to electrophoresis tank. Positive samples were sequenced by Mega ۵.Results: ۸ HPV+ samples include of ۳ HPV+۶ and HPV+۱۶ were found . HPV۶ is the cause of genital warts that can spread by skin contact or oral- sexual behavior. ۳ positive samples were found in women and the others were in men. (۲% more in men) . People between ۳۰ to۴۵ are more sensitive for HPV than the other group. People up to ۶۰ years old are sensitive too. All the samples were collected from different cities of Iran but most of the positive samples were found in Tehran and Islam Shahr.Conclusion: These data confirm that HPV infection with high risk types (۶, ۱۶) could be one of the risk factors for oral cancer and it can spread by genital warts.

کلیدواژه ها:

"Human papilloma virus" ، "HPV-۶" ، "HPV-۱۶" ، "squamous cell Carcinoma" ، "Polymerase Chain Reaction (PCR)" ، کارسینوم سلول سنگفرشی ، ویروس پاپیلومای انسانی ، واکنش زنجیره ای پلیمراز