Cytotoxicity Effect of Recombinant Outer Membrane Inflammatory Protein (oipA) of Helicobacter pylori on a Breast Cancer Cell line

Objective: Breast cancer is one of the leading causes of death in women worldwide. Conventional treatments use cytotoxic drugs which have high numbers of side effects. Currently pharmacologists are searching for novel drugs with fewer side effects and maximum efficiency as breast cancer treatment. The aim of the current study is to clarify the cytotoxicity effect of the recombinant outer membrane inflammatory protein (oipA) of Helicobacter pylori (H. pylori) on a breast cancer cell line. Methods: We purified recombinant H. pylori oipA by Ni–NTA affinity chromatography. Breast cancer cells (۴T۱) were treated with different concentrations of recombinant oipA for various lengths of time. Cell viability was evaluated by the viability assay (MTT test). Results: SDS-PAGE analysis showed the expression of an approximately ۳۴۰۰۰ dalton protein. Statistical analysis showed oipA toxic effects on ۴T۱ cells at a concentration of ۲۵۰ µg/ml after ۲۴ h. Conclusion:  These findings suggested that oipA had a direct toxic effect on a breast cancer cell line (۴T۱) in vitro. The oipA protein might be a new tool for future therapeutic strategies in cancer immunotherapy.

کلیدواژه ها:

Breast Cancer ، Viability ، ۴T۱ cell ، Outer inflammatory protein A ، سلولهای ۴T۱ ، بقای سلولی ، پروتئین نوترکیب غشای خارجی التهابی ، سرطان سینه