Dendrosomal curcumin Induced Apoptosis by Suppression of Pluripotency Genes in ۵۶۳۷ Bladder Cancer Cells

Objective: The anti-cancer properties of curcumin, a poliphenol extract from the rhizome of curry, has been confirmed by many investigators. However, low levels of uptake, tissue distribution and rapid metabolism has limited its application as an anti-cancer drug. This study is aimed at increasing curcumin's water solubility due to a biodegradable, neutral and non-toxic micellar nano-carrier called dendrosome. This study intends to evaluate the role of dendrosomal-curcumin (DNC) in bladder cancer cell growth. Methods: We performed the MTT assay, flow cytometry and Annexin V-FLUOS (as an apoptosis detection kit) to evaluate cell death. The genetic mechanism of DNC-induced apoptosis was accomplished by a study of the relative expressions of OCT۴A, OCT۴B۱, SOX-۲ and Nanog using real-time PCR. Results: DNC-induced cell death complied with a time and dose-dependent paradigm in the ۵۶۳۷ cell line. Cell cycle analysis revealed that the number of cells increased in pre-G۱ and gradually decreased in G۱ and S phases. This showed the inhibitory property of dendrosomal-curcumin on DNA synthesis. Data from real-time PCR determined that expressions of OCT۴A, OCT۴B۱, SOX-۲ and Nanog could be related to ۵۶۳۷ cancer cell growth. Dendrosomal-curcumin significantly suppressed mRNA expression of the above mentioned genes (pConclusion: The data showed that DNC induced apoptosis by suppression of pluripotency genes in ۵۶۳۷ bladder cancer cells, which confirmed the useful characteristic of nano-drug in bladder cancer therapy

کلیدواژه ها:

Bladder cancer ، Dendrosome ، Curcumin ، Cell-programmed death ، Pluripotency genes ، سرطان مثانه ، دندروزوم ، کورکومین ، مرگ برنامهریزی شده سلولی ، ژنهای پرتوانی