Evaluation of Cisplatin and Cisplatin-loaded Magnetic Iron Oxide Nanoparticles on BCL۲ and BAX genes in the Breast Cancer T۴۷D Cell Line

Objective: Breast cancer is the second leading cause of cancer death in women. Cisplatin is a traditional cancer drug commonly used in chemotherapy for killing cancer cells. Modulation at the mRNA levels of apoptotic related genes often correlate with the sensitivity of various types of cancer cells to chemotherapeutic agents. Nanoparticulate drug delivery systems are being developed to effectively deliver smaller doses of chemotherapeutic agents and control drug distribution in the body. In this study, we evaluate the expressions of BCL۲ and BAX genes in T۴۷D treated with cisplatin and cisplatin nanoparticles, which can result in a new approach to breast cancer therapy. Methods: In this study, we treated T۴۷D cells with different concentrations of cisplatin and cisplatin nanoparticles at ۴۸ h. The IC۵۰ was determined. We extracted RNA by using RNX solution, after which cDNA was synthesized. The precise primers for the BCL۲, BAX and TBP genes were designed by specific software. The quantity of BCL۲ and BAX gene expression compared to TBP gene (reference gene) was analyzed using real-time PCR.  Results: BCL۲ and BAX gene expression levels in T۴۷D cells treated by cisplatin were ۰.۷ (BCL۲) and ۱.۴۸ (BAX); in T۴۷D cells treated with cisplatin-loaded nanoparticles, the gene expressions were ۰.۰۳ (BCL۲) and ۲.۴۱ (BAX). Conclusion: In this study, the results have shown that cisplatin-loaded nanoparticles are effective anticancer agents. Cisplatin nanoparticles induce apoptosis in human breast cancer cell lines. We have shown that cisplatin nanoparticles strongly increased cytotoxicity in comparison to the free drug in the T۴۷D cell line.

کلیدواژه ها:

Breast Cancer ، Cisplatin ، Nanoparticle ، Programmed Cell Death Gene ، سرطان پستان ، نانوذره ، سیسپلاتین ، ژنهای مرگ سلولی برنامهریزی شده