Relationship between idiopathic female infertility with KISS۱receptor gene mutation in northern Iranian women

Background: The kiss peptins and its receptor G protein coupled receptor (GPR۵۴) or KISS۱ receptor system are being described as key signaling molecules for reproductive function in animal models and humans. They play essential roles in regulation of the hypothalamic- pituitary- gonadal (HPG) axis and the onset of puberty and fertility. Objective: This study was performed to delineate the association of T۳۰۵C (Leu ۱۰۲ Pro) KISS۱ receptor gene mutation with idiopathic female infertility in Iranian women. Methods: In this study, ۱۴۰ healthy women with at least one child and no history of infertility and abortion and ۱۳۰ idiopathic infertile women were recruited for this study.  By using allele specific PCR (AS-PCR) method, the allele and genotype frequencies among infertile and healthy women were determined. Results: The gene frequencies of the ۳۰۵ T and C allele of the KISS۱ receptor were ۴۵% and ۵۴% among infertile women and ۵۰% and ۵۰% among healthy controls, respectively. The distribution of genotype frequencies in the patients and controls was as follows: TT (Leu/Leu) was ۱۵% and ۰%, TC (Leu/Proline) was ۶۰% and ۱۰۰% and CC (Pro/Pro) was ۲۴% and ۰% respectively. Structural analysis was performed using the MedCalc program (version ۱۲). Our results suggests that significant association were not observed in genotype (P=۰.۸) and allelic (P=۰.۶) distribution between cases and controls. Conclusions: The data presented show that mutant allele C  is not a risk factor for infertility, suggesting that the presence of KISS۱ receptor T۳۰۵C mutation is probably not associated with idiopathic female infertility in this population (P>۰.۰۵).

کلیدواژه ها:

Infertility ، KISS۱ receptor ، Mutation