Study of Relationship between Polymorphism in Estrogen Response Element (ERE) in Promoter Region of C۳ Gene and Spontaneous Recurrent Abortion

Background: Miscarriage is one of the most common pregnancy complications for which various causes have been defined, such as genetic factors, infectious, metabolic, endocrine systemmal function and immune system undesired responses. The early development of embryo occurs in oviduct and uterine tube from which some factors such as growth factors, glyco-proteins and factors those stimulate development of embryo are secreted. The ETF۳ embryotrophic factor which is a complex of C۳ complements and its derivatives i.e., iC۳b, enhances the development of trophectodermas a consequence of which expression of relevant genes are affected embryo. There are various response elements in C۳ gene promoter region such as, estrogen response regions (ERE). Steroids such as estrogen and progesterone are secreted in early steps of embryonic period along with C۳ secretion and cause increase in C۳ expression through interaction with regulatory elements in promoter region of this gene. In this study the polymorphism in ERE regions of C۳ gene promoter was investigated in women suffering from recurrent miscarriage. Materials and methods: In this study, assuming that polymorphism in ERE regions is correlated with recurrent miscarriage during early months of pregnancy, ۴۰ blood samples were collected from female patients admitted to an Infertility Clinic, Isfahan, Iran. DNA was extracted, amplification of regions harboring ERE with a pair of specific primer was done using Polymerase Chain Reaction-Single Strand Chain Polymorphism (PCR-SSCP) for studying possible polymorphisms in this region. Results and conclusion: The results indicated a specific symptomless infertility among the women, however there was no correlation between the ERE polymorphism and symptoms in control and cases.

کلیدواژه ها:

Spontaneous ، Recurrent Abortion ، Complement ۳ ، Estrogen Response Element