Analysis of MnSOD and VEGF genes polymorphisms in Diabetic Retinopathy

Background:Diabetic retinopathy (DR) is a sight-threatening microvascular complication of diabetes in which the vascular endothelium is damaged due to oxidative stress and inflammation, and vitreous VEGF concentration becomes elevated. The aim of the present study was to assess the association of DR with genetic variations of the MnSOD, a major antioxidant enzyme, and VEGF, an important mediator of neovascularisation, in northern Iran. Methods: ۷۰ patients with DR and ۷۰ healthy control subjects matched for age and sex was recruited for this study. PCR-based RFLP assay was used to determine the genotypes of MnSODA۱۶V and VEGF+۴۰۵ C/G polymorphisms. Results and Conclusions:A higher frequency of the AV genotype (۷۱.۴۳%) of the MnSODA۱۶V polymorphism was found in the patients compared with controls which had a ۸.۳۳-fold increase in risk of DR (OR= ۸.۳۳, ۹۵% CI= ۲.۵۶-۲۷.۱۳, P= ۰.۰۰۰۴). The frequency of GG, GC, and CC genotypes of VEGF +۴۰۵ C/G polymorphism in controls were ۴۲.۸۶%, ۴۵.۷۱% and ۱۱.۴۳%, respectively, while in DR patients were ۱۸.۵۷%, ۴۸.۵۷%, and ۳۲.۸۶%, respectively.The +۴۰۵C allele was considered as a high risk factor of DR (OR= ۲.۵۵, ۹۵% CI= ۱.۵۷-۴.۱۴, P= ۰.۰۰۰۱). In conclusion, It is suggested that the MnSODA۱۶V and the VEGF+۴۰۵ C/G polymorphisms may be associated with the risk of DR in northern Iran.

کلیدواژه ها:

Diabetic retinopathy ، gene polymorphism ، VEGF andMnSOD