Genetic diversity of some thornless blackberry genotypes using ISSR molecular markers

Genetic diversity in blackberries is crucial for improving quality and yield. This study evaluates thornless blackberry genotypes using ۲۶ morphological traits and ۱۹ inter simple sequence repeats (ISSR) markers to assess diversity and genetic relationships. A total of ۲۸ blackberry genotypes, including both thorny and thornless types, were analyzed. Fourteen ISSR primers were selected from a pool of ۱۹ based on their capacity to generate polymorphic bands. The findings highlight the efficiency of ISSR markers in distinguishing thorny and thornless blackberry genotypes at the subgenus level, effectively differentiating chimera-derived thornless samples. A total of ۴۰۶ bands were produced, of which ۴۰۲ were polymorphic. The average percentage of polymorphic bands for each primer in this experiment was ۹۸.۹۸%, and the highest polymorphic information content (PIC) was associated with ISSR ۱۶, which had a value of ۰.۳۵. The findings highlight the efficiency of ISSR markers in distinguishing thorny and thornless blackberry genotypes at the main genotype groups: the first linked to initial thornless generations of Rubus laciniatus, and the second comprising crosses related to the Merton cultivar. Overall, significant genetic diversity among blackberry cultivars suggests valuable applications in breeding and improvement programs.

کلیدواژه ها:

Rubus laciniatus ، merton thornless ، Genetic diversity ، Molecular marker