Decreased expression of miRNA-۲۰۰a and miRNA-۲۲۳-۳p in endometriosis cases during the secretory phase of the menstrual cycle: insights from a comprehensive case-control study with analysis of molecular biomarkers and their potential role in disease-induced infertility.

Background: Endometriosis (EM) is a condition that causes infertility with decreasing uterine receptivity. It is reported that it affects about ۲۰-۲۵% of all infertile women. Some genetic markers play a crucial role in pathogenesis and infertility. Objective: This study investigates the role of miR-۲۰۰a and miR-۲۲۳-۳p in embryo implantation and their association with EM-related infertility. Materials and Methods: In this case-control study, ۳۶ women who referred to the Center for Research on Reproductive Health and Infertility of Babol University of Medical Sciences and Fatemeh Al-Zahra Infertility Specialized Treatment Center in Babol, Iran between June ۲۰۲۲ and July ۲۰۲۳ were evaluated. Participants were divided into ۲ EM and control groups (n = ۱۸/each). Endometrial samples were collected from participants between ۱۷th and ۲۴th days of their menstrual cycle. Histopathological examination (hematoxylin and eosin and periodic acid schiff) was performed to confirm the secretory stage, and miR-۲۰۰a and miR-۲۲۳-۳p expressions were analyzed by quantitative reverse transcriptase-polymerase chain reaction. Results: Histological analysis confirmed that both groups were in the secretory stage. Additionally, miRNA expression results showed a significant decrease in the miR-۲۰۰a and miR-۲۲۳-۳p expression levels in EM group compared to control group. The expression level of miR-۲۲۳-۳p and miR-۲۰۰a in the eutopic endometrial tissue of women with EM was notably lower than those in the control group. Conclusion: Our results suggest that miR-۲۰۰a and miR-۲۲۳-۳p are involved in the EM pathogenesis, while other genes and signaling pathways are probably involved in the implantation failure.   This article has been extracted from M.Sc. Thesis. (Yasaman Nazari Hagh)

کلیدواژه ها:

Endometriosis ، miRNA ، Implantation ، Signaling pathways ، Biomarkers ، Infertility ، Menstrual cycle. ، اندومتریوزیس ، miRNA ، لانه گزینی ، مسیرهای سیگنالینگ ، بیومارکرها ، ناباروری ، سیکل قاعدگی.