Propagation Properties of a New Human Diploid Cell Line, RAZI-HDC, and Its Suitability as a Candidate Cell Substrate for Respiratory Syncytial Virus Vaccine Production in Comparison to MRC-۵

Respiratory syncytial virus is a common cause of infection of the respiratory tract in infants, older adults, individuals with heart and lung disease, and immunocompromised patients. The disease causes between ۱۰۰,۰۰۰ and ۲۰۰,۰۰۰ infant deaths annually. Several vaccine platforms have been introduced for RSV vaccine production. In this study, a local diploid cell line, RAZI-HDC, derived from human fetal lung cells, was used for RSV virus propagation regarding to study live-attenuated vaccine, and was compared to the MRC-۵ cell line. The total cells per ۲۵cm۲ flask were ۴۴.۰ ± ۲.۶ *۱۰۵ and ۴۱.۶۶±۲.۰۸ *۱۰۵ for MRC-۵ and RAZI-HDC, respectively. The maximum cell-specific growth rate of RAZI-HDC was ۳۱۶.۶۶±۲۰.۸۱, while that of MRC-۵ was only ۳۴۰±۲۶.۴۵. The maximum cell division number of RAZI-HDC was ۱.۲۴±۰.۰۷ in comparison to the MRC-۵, with a maximum cell division number of ۱.۳۲±۰.۰۸. Both cell substrates achieved maximum cell density ۵ days after starting the culture. The complete cytopathic effect of RSV in RAZI-HDCR-HDC was observed after four days, which indicates the sensitivity of these cells to RSV. The virus productivity in RAZI-HDC cells (۲.۴۶۸۵) was not significantly different from that in MRC-۵ cells (۲.۵), as determined by a two-tailed t-test (p=۰.۷۸). The results showed that both cell substrates have the same function for RSV propagation. Diploid cell lines like MRC-۵ and RAZI-HDC are preferred for vaccine manufacturing as they are of human origin and have a stable karyotype. This is a significant advantage, as it helps ensure the safety of the final vaccine product if these cells are used to make viral vaccines that require virus amplification. The ability of RAZI-HDC cell line in supporting the RSV replication, were assessed and found to be equivalent to those of MRC-۵. Specifically, the maximum virus productivity in RAZI-HDC cells (۲.۴۶۸۵ log TCID۵۰/mL) was not significantly different from that in MRC-۵ cells (۲.۵ log TCID۵۰/mL), as determined by statistical analysis. Using a locally developed cell line like RAZI-HDC can be somewhat more cost-effective than relying on imported cell substrates.

کلیدواژه ها:

Keywords: RSV ، Propagation ، RAZI- HDC ، MRC-۵ cell line ، vaccine