The impact of Polymorphisms of rs۶۸۹ and rs۷۵۷۱۱۰ in Familial Type ۲ Diabetes: A Case-Control Study in the Iranian Population

Objective: Understanding the genetic basis of type ۲ diabetes mellitus (T۲DM) is essential in getting its etiology and designing effective preventive strategies. Evaluation of the association between the gene polymorphisms rs۶۸۹A/T (INS) and rs۷۵۷۱۱۰C/A (ABCC۸) and the susceptibility to T۲DM within a group of individuals diagnosed with T۲DM in Iran. Materials and Methods: Blood samples were used for DNA extraction (۲۰۰ with T۲DM and ۲۰۰ healthy controls). The quality and quantity of extracted DNA were assessed via ultraviolet spectrophotometry at ۲۶۰ nm and ۲۸۰ nm wavelengths. To identify specific alleles, primer sequences were manually designed using Primer۳Plus, and the genotypes of rs۶۸۹ (A>T) and rs۷۵۷۱۱۰ C>A were determined through ARMS-PCR. Statistical analysis was conducted using GraphPad Prism version ۸. Results: For rs۶۸۹ individuals with the genotype (AA) were found to have a higher likelihood of developing diabetes (P= ۰.۰۰۱). Additionally, the frequency of the A allele was found to be higher in the patient group (۰.۱۳) compared to the control group (۰.۰۵). For rs۷۵۷۱۱۰ individuals with the genotype (CC) were found to have a higher likelihood of developing diabetes (P< ۰.۰۰۱). Additionally, the frequency of the C allele was found to be higher in the patient group (۰.۵۷) compared to the control group (۰.۳۹). Conclusion: This study found that the frequency of AA and AT genotypes of rs۶۸۹ and CC and CA genotypes of rs۷۵۷۱۱۰ are associated with T۲DM risk.

کلیدواژه ها:

Diabetes ، ARMS-PCR ، rs۶۸۹ ، rs۷۵۷۱۱۰