Therapeutic Potentials of Persea americana Peptide: In silico and Experimental studies

Objective: Salmonella infections constitute a growing healthcare concern ranging from gastroenteritis to severe invasive diseases that require hospitalization. Persea americana is valued for its high nutritional content and health benefits. This study evaluated the therapeutic effects of Persea americana derived seed peptide (PASP) on Salmonella-infected Wistar rats. Methods: Peptide from the plant material was obtained via aqueous extraction. Susceptibility testing of PASP against the test multi-drug resistant Salmonella typhi was performed using standard microbiological assays. The constituents in PASP were measured via high performance liquid chromatography (HPLC) analysis, and virtually screened against DNA gyrase B. Therapeutic effect of PASP was evaluated in-vivo using murine model, comprising seven different groups (n=۳). Results: PASP elicited potent activity against the tested organism producing inhibition zone of: ۲۲ mm at ۱۰۰ mg/ml, and MIC value of ۲۵ mg/ml. The pharmacodynamics of PASP revealed a time dependent decline in microbial cells. Amongst the constituents of PASP, Phenylalanine, Cystine, Histidine, Aspartic acid, Glutamic acid had the highest binding scores, with the free binding energy of these compounds superior to Ofloxacin. PASP had appreciable therapeutic effect on the S. typhi infected rats via alleviating signs and symptoms of infection, modulating hematological parameters PCV (۴۴.۰۰±۰.۵۷%), WBC (۶.۹۳±۲.۰۸x۱۰۹/L), Lymphoctyes (۸.۴۶±۴.۰۸%); and had no pronounced detrimental impact on the rats’ biomarkers ALP (۴۴.۳۳±۳.۱۷ U/L), AST (۱۸۴.۶۶±۴.۰۵ U/L), ALT (۱۰۸.۳۳±۱۳.۱۷ U/L) (group administered with ۲۵ mg/ml PASP). Discussion: These findings provide substantial evidence on the safety and anti-Salmonella efficacy of bioactive peptide from P. americana seeds.

کلیدواژه ها:

Persea americana seeds ، peptide ، Salmonella typhi ، antimicrobial resistance ، DNA gyrase ، virtual screening