Investigation of Non-Coding RNA-Related Autophagy Alterations in Drug-Resistant Multiple Myeloma Plasma Cells

Background & Objective: Multiple myeloma (MM) drug resistance is thought to be caused by the development of protective autophagy. This work aimed to assess the non-coding RNA (ncRNA) autophagy-related alterations in drug-resistant (DR) myeloma cells.Methods: DR Plasma cells were extracted from the bone marrow of DR patients referred to Baghai ۲ Hospital in Ahvaz, Iran. The cells were grown in RPMI-۱۶۴۰ media containing ۱۰% FBS and ۱% Pen/Strep and incubated at ۳۷˚C and ۵% CO۲. After six passages, the plasma cells were precisely isolated and utilized as DR cells. The U۲۶۶B۱ cell line (IBRC C۱۰۱۴۸) was grown in the RPMI-۱۶۴۰ media containing ۱۰% FBS and ۱% Pen/Strep and utilized as drug-sensitive (DS) cells. The relative expression of the genes was determined using the Real-time PCR method. Statistical analysis of the data was performed using GraphPad Prism ۸ software.  Results: When the DR cells were compared to the DS cells, there was a notable increase in the expression of ULK۱ and LC۳B. However, expression of P۶۲ in the DR plasma cells showed a significant decrease compared to the DS plasma cells. The miR-۱۲۹۷ level was considerably higher in the DR cells than in the DS cells. Although, there was no statistically significant difference in the expression of miR-۲۶a-۵p between the DS and DR cells. The DR cells exhibited a statistically significant increase in the expression of MALAT۱ and SNHG۶.Conclusion: Drug resistance in MM cells may result from overexpression of non-coding RNAs involved in autophagy.

کلیدواژه ها:

Multiple Myeloma ، Drug resistance ، autophagy ، non-coding RNA ، Plasma cell