In vitro propagation of Physalis alkekengi L. using axillary buds

Objective: Chinese lantern (Physalis alkekengi L.) is an important medical herb due to its physalin. This plant is widely used to treat various diseases. Therefore, developing a repeatable micropropagation method for this plant would be valuable.Methods: In this study, shoot tips and axillary buds of Chinese lantern were treated with different growth regulators (IAA, NAA, BAP, GA۳, and IBA) with various concentrations. In another experiment, the effect of salt concentration on the MS medium was investigated.Results: The results showed that using axillary buds as an explant in the full-strength MS, supplemented with ۲ mg/L BAP and ۰.۵ mg/L IAA, resulted in a higher number of shoots per explant (۳±۱.۲۲) and the highest number of nodes (۱۳±۱.۲۲) and stem length (۸.۰۴±۱.۹۲). Compared with other strengths of MS salts, the full-strength MS medium, supplemented with ۲ mg/L BAP and ۰.۵ mg/L IAA, or ۰.۵ mg/L BAP alone produced the highest number of shoots per explant, number of nodes, and stem length. However, the MS salt supplemented with a low concentration of BAP (۰.۵ mg/L) alone looks economically feasible. Decreasing the salt concentration of the basal medium decreased all micropropagation characteristics and in most cases, the decrease was statistically significant. The ddition of GA۳ to the combination of ۲ mg/L BAP and ۰.۵ mg/L IAA, significantly improved stem elongation at the concentration range of ۰.۵ – ۱.۵ mg/L The number of roots was higher (۲±۰۰) after ۳۰ days on the MS basal medium when supplemented with ۱ and ۲ mg/L NAA or ۲ mg/L IAA. The well-rooted plantlets were isolated and transplanted to paper cups for hardening and the well-established plants were transferred to the field for acclimatization.Conclusion: In conclusion, our results showed that the full strength of MS medium, supplemented with ۲ mg/L BAP plus ۰.۵ mg/L IAA and ۰.۵–۱.۵ mg/L GA۳ improved micropropagation characteristics of P. alkekengi.

کلیدواژه ها:

axillary buds ، Chinese lantern ، Micropropagation ، organogenesis ، Physalis alkekengi