Development of a honey-based bilayer wound dressing: a novel strategy for effective wound recovery

To address the limitations of traditional single-layer wound dressings, bilayer dressings have emerged as a viable alternative for effective and sustained wound management. This study presents a novel bilayer wound dressing aimed at enhancing wound healing. The dressing combines a polyacrylic acid (PAAc)-honey (Hny) sponge layer with an electrospun nanofiber layer composed of Keratin (Kr), Hny, and vascular endothelial growth factor (VEGF). Comprehensive evaluations assessed the physiochemical properties, VEGF release profiles, and biological responses, including cytocompatibility and cell migration. The results demonstrated favorable mechanical properties and significant water absorption. Moreover, the PAAc-Hny/Hny-Kr-VEGF exhibited sustained VEGF release over seven days, promoting enhanced angiogenesis and improved cellular response. In vivo experiments in rat models, revealed significantly improved healing rates, reduced inflammation, and increased collagen deposition in wounds treated with the PAAc-Hny/Hny-Kr-VEGF dressing. Histological analysis confirmed complete wound closure and regeneration of skin appendages. This bilayer dressing shows significant promise for effective wound management in clinical applications.

کلیدواژه ها:

Honey ، Bilayer wound dressing ، Polyacrylic acid ، Sponge ، Electrospun ، Nanofiber