Rosmarinic acid attenuated inflammation and apoptosis in folic acid-induced renal injury: Role of FoxO۳/ NFκB pathway

Objective(s): Rosmarinic acid (RA) is a herbal compound with various antioxidant and anti-inflammatory effects. This study aimed to explore the anti-inflammatory and anti-apoptotic properties of RA in folic acid-induced renal injury.Materials and Methods: Thirty-six male C۵۷/BL۶ mice were randomly divided into six groups (N=۶): Control (received normal saline), NaHCO۳ (received NaHCO۳ as folic acid solvent), FA (received folic acid (FA)(IP) to induce renal injury), RA (received ۱۰۰ mg/kg RA), RA۵۰-FA (received ۵۰ mg/kg RA solution after folic acid injection), and RA۱۰۰-FA (received ۱۰۰ mg/kg RA after folic acid injection). For ten days, the treatment groups received RA by gavage. The effects of RA were assessed using H & E staining, biochemical tests, western blotting, and ELISA in the kidney tissues of the mice. Real-time RT-PCR was also performed to evaluate the expression changes of renal genes.Results: Our data showed that treatment by RA led to the over-expression of FoxO۳ (P<۰.۰۵) and decrease in NFκB levels (P<۰.۰۱ and P<۰.۰۵) and expression of TNFα (P<۰.۰۵) and IL۶ (P<۰.۰۰۱ and P<۰.۰۱). Other evaluations showed a decrease in p۵۳ (P<۰.۰۱ and P<۰.۰۰۱), Bax/Bcl-۲ ratio expression (P<۰.۰۱ and P<۰.۰۵), and Caspase-۳ level (P<۰.۰۱ and P<۰.۰۵) compared to the folic acid group. Histological and biochemical results also confirmed the attenuation of tissue damage.Conclusion: This study revealed that RA’s positive effects on folic acid-induced renal injury might result from the involvement of the FoxO۳/NFκB pathway, thereby suppressing inflammation and apoptosis.

کلیدواژه ها:

Apoptosis ، folic acid ، Inflammation ، Kidney Injury ، Rosmarinic acid