Yavar-۷۰A, a novel water-in-oil adjuvant: A potency study in HPV-۱۶E۷d vaccine model

Objective(s): Adjuvants are some of the most important components used for vaccine formulation. In addition, the efficacy of vaccines is highly dependent on the nature of the adjuvants used. Therefore, new adjuvant formulations may help develop more potent vaccines. In the present study, the potency of an in-house and water-in-oil adjuvant (Yavar-۷۰A) was compared with Montanide ISA ۲۰۶ and Montanide ISA ۲۶۶ in an HPV-۱۶E۷d vaccine model. Materials and Methods: Three HPV-۱۶ E۷d vaccines were formulated using three different adjuvants, Montanide ISA ۲۰۶, Montanide ISA ۲۶۶, and Yavar-۷۰A, with standard protocols. Afterward, each formulation containing ۱۰ μg of the E۷d protein was administered thrice at two-week intervals to C۵۷BL/۶ mice. Serum levels of IFN-γ and IL-۴ cytokines secreted from spleen cells, total IgG, and specific IgG۱ and IgG۲a isotypes were assessed using ELISA two weeks after the last immunization. Lymphocyte proliferative responses were also evaluated using the BrdU method.Results: The results indicated that the vaccine formulated using the Yavar-۷۰A adjuvant showed the highest lymphocyte proliferation responses compared with other groups and higher IFN-γ cytokine release compared with that formulated using Montanide ISA ۲۰۶. However, the vaccine formulated using Montanide ISA ۲۰۶ induced the highest total IgG responses compared with other groups. Importantly, the vaccine formulated using Yavar-۷۰A decreased IL-۴ secretion compared with other vaccinated groups.Conclusion: The present study demonstrated that Yavar-۷۰A induces cellular and humoral immunologic parameters against the HPV-۱۶ E۷d vaccine model comparable to commercialized oil-based adjuvants.

کلیدواژه ها:

HPV-۱۶-E۷d ، IFN-γ ، Oil adjuvants ، Vaccine formulation ، Yavar-۷۰A