Investigation the interaction between hsa-miR-۱۵۳-۳p and BCL-۲ gene in cervical cancer progression via bioinformatical analysis

The most frequent gynecological cancer that harms women's health worldwide is cervical carcinoma. Cervical carcinoma has the fourth-highest fatality rate of any type of cancer. Cervical cancer is mostly associated with high-risk human papillomavirus infection; having several sexual partners is a secondary risk factor. The following are additional risk factors for cervical carcinoma: starvation, smoking, and ill health. Numerous techniques are employed to treat cervical cancer and raise the likelihood of survival. Chemotherapy, radiation, and surgery make up the majority of the current treatment. Nonetheless, there is a yearly rise in both the prevalence and recurrence rates of cervical cancer. Clarifying the molecular pathways underlying the onset and spread of cervical carcinoma is therefore necessary in order to identify possible therapeutic targets and identify cervical cancer biomarkers. One crucial biological process that happens in response to developmental signals or cellular stress is apoptosis. Cancer develops primarily as a result of impaired apoptosis. The B-cell lymphoma ۲ (BCL-۲) protein family exhibits either pro- or anti-apoptotic properties. MATERIALS AND METHODS: A wide variety of coding transcripts have been profiled and their underlying mechanisms of action have been mapped because to developments in high-throughput profiling techniques and the accessibility of public data sets like The Cancer Genome Atlas Program (TCGA). miRNAs control a wide variety of processes in cell biology. Acquiring the miRNA target genes is useful in suppressing these pathways and can aid in the understanding of cancer regulation and gene therapy. In this effort, we evaluated TCGA RNA-seq data and projected gene expression patterns after miRNA target gene prediction using the mirwalk database. RESULTS AND DISCUSSION: Based on the results, hsa-miR-۱۵۳-۳p targets BCL-۲, which has a high score. Furthermore, the analysis of the TCGA data revealed a significant decrease in this gene's expression in the tissue from cervical carcinomas. CONCLUSION: Predictions indicate that upregulating hsa-miR-۱۵۳-۳p expression results in downregulation of BCL-۲ expression in cervical carcinoma. Furthermore, downregulation of BCL-۲ expression is expected to contribute to the progression of cervical carcinoma in addition to serving as a biomarker for the disease.

کلیدواژه ها:

cervical carcinoma ، hsa-miR-۱۵۳-۳p ، BCL-۲