Dendrosomal nanosolanine affects the PI۳K pathway in colorectal cancer

Introduction Colorectal cancer (CRC) is the third most common type of cancer in the world. CRC can caused by mutations, and based on their origin, CRC can be classified as familial, inherited, and sporadic. The PI۳K pathway is involved in cell proliferation and survival. Alterations in this pathway could play an important role in cancer cell proliferation. Alteration of PIK۳CA is one of the most common mutations in CRC. PTEN downregulates the PI۳K pathway. Loss of function of PTEN is related to poor survival in metastasis. According to the stage of CRC, there are different treatment methods. Surgery, chemotherapy, radiation therapy, and targeted therapy are some of these methods. Many studies have been conducted on targeting the PI۳K/Akt pathway in colorectal cancer. Due to alterations in this pathway, this pathway and investigating its role have been considered. Previous studies have shown the anticancer effects of solanine and its effect on the PI۳K/Akt pathway. Furthermore, applying the dendrosomal nanosolanine (DNS) in chronic myeloid leukemia cell lines was more effective in inhibiting this pathway than solanine. Methods In this study, the purpose was to investigate the effects of DNS on the PI۳K/Akt pathway in colorectal cancer. In this regard, HCT۱۱۶ cells were treated with this compound. After ۴۸ hours of treatment, RNA was extracted. To find out the extracted RNA concentration, the nanodrop was applied. cDNA was synthesized from extracted RNA. cDNA quality was evaluated using PCR. To assess the expression of the PI۳K pathway-related gene, real-time PCR was used. In order to assess gene expression on the protein level, western blotting was conducted. Results PCR data confirmed the quality of synthesized cDNA. Based on real-time PCR obtained data, DNS treatment can cause alteration in expression of the PI۳K pathway-related gene (PTEN). Western blotting confirmed real-time PCR data as well, and PTEN was increased after DNS treatment. Conclusion Our results showed significant alteration in the expression of PTEN on both the mRNA and protein levels. Due to the inhibitory effect of PTEN on the PI۳K pathway, this finding supports the idea that DNS can suppress this pathway in the HCT۱۱۶ cell line. Deregulation of this pathway has an important role in cell proliferation in CRC. It can be said that DNS may reduce HCT۱۱۶ proliferation by affecting the PI۳K pathway.

کلیدواژه ها:

Colorectal cancer ، PI۳K pathway ، Dendrosomal nanosolanine