Assessment of Antibacterial Effects of Ethyle Octahydrospiro[indene-۲,۳'-pyrrolizidine]- ۲' Carboxylate Against Escherichia coli and Staphylococcus aureus bacteria

Objective: The emergence of resistance in bacteria, and the existence of various types of infection and contamination in the hospital and society is the basis for research to find new antibacterial compounds.  Materials and methods: EthyleOctahydrospiro[indene-۲,۳'-pyrrolizidine]-۱,۳-diones-pyrrolizidine]-۲'carboxylate (۵) was synthesized during one-pot reaction of ninhydrin, proline and ethyl acrylate in ethanol solvent. The antibacterial effect of the synthesized derivative on the mentioned bacteria was investigated by surface culture methods in Mueller Hinton agar culture medium and microdilution, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for the synthesized derivative.. Findings: The analysis of the results showed that the MIC and MBC values of the newly synthesized compound against Staphylococcus aureus (S. aureus) bacteria were ۱.۵۶ and ۳.۱۲ μg/mlL, respectively. In addition, the MIC and MBC values of the synthetic compound against Escherichia coli (E. coli) were ۰.۳۹ μg/mlL and ۰.۷۸ μg/mlL, respectively. MIC and MBC values of standard gentamicin against E. coli bacteria were ۳.۲ and ۶.۴ μg/mL, respectively. MIC and MBC values of standard gentamicin against E. coli bacteria were ۳.۲ and ۶.۴ μg/mlL, respectively. Conclusion: The derivative synthesized with indane and pyrrolizidine pharmacophores showed good antibacterial power against S. aureus, E. coli bacteria. Conclusion: The antimicrobial test results revealed that the synthesized compound at an equivalent concentration exhibited a higher inhibitory impact on the Gram-negative E. coli bacteria than Gram-positive S. aureus. However, it demonstrated a more potent effect on the Gram-negative bacteria. The compound causes the immediate death of Gram-negative bacteria by destroying or disrupting the function of the outer membrane. This combination could be effective in treating infections caused by Gram-negative bacteria.

کلیدواژه ها:

chiral azomethylene ylide ، ۳ dipolar cyclization ، Escherichia coli ، indane