Epigenomic profiling in Cancer: Techniques and Therapeutic Implications

Understanding the control of gene expression and its consequences for cancer biology is greatly aided by the field of epigenomics, which is the study of epigenetic changes throughout the genome. Epigenetic modifications, including DNA methylation, histone modifications, chromatin remodeling, and non-coding RNA regulation, affect gene activity without altering the DNA sequence, in contrast to genetic mutations. These alterations have a critical role in controlling gene expression, which affects cellular functions such as growth, differentiation, and death. Epigenetic modifications play a major role in cancer by causing dysregulated gene expression, which silences tumor suppressor genes, activates oncogenes, and encourages genomic instability. For instance, hypermethylation of CpG islands at the promoters of tumor suppressor genes, such as p۱۶INK۴a and BRCA۱, results in the transcriptional repression of these genes. In contrast, global hypomethylation activates oncogenes and causes chromosomal instability. Gene expression and the development of cancer are also highly impacted by histone alterations and chromatin remodeling. The methods for characterizing epigenetic changes, such as DNA methylation, histone modifications, chromatin accessibility, and non-coding RNA interactions, are explained in this article. It emphasizes how important these methods are for identifying the epigenetic changes that contribute to the development and progression of cancer. By addressing therapeutic implications and new treatments like DNA methylation and histone deacetylase inhibitors, this review closes the gap between basic epigenomic changes and their possible application in clinical practice. This study intends to improve cancer detection, prognosis, and treatment by providing a thorough grasp of epigenomic profiling, opening the door for more individualized and successful therapeutic approaches.

کلیدواژه ها:

epigenomics ، Cancer Biology ، DNA methylation ، Histone Modifications ، Chromatin Remodeling ، Precision Oncology