Assessment of the probiotic isolates features found in breast milk

The probiotics in breast milk can help children restore optimal growth. This study aimed to separate and identify probiotic microorganisms from human milk and evaluation of vitamin B production by these microorganisms. Biochemical tests were used to identify nine aerobic and ۸۱ microaerophilic isolates from ۳۴ breast milk samples; Probiotic traits were characterized for auto-aggregation, co-aggregation, hydrophobicity, acidity tolerance, lysozyme, bile salts, and papain treatment. Furthermore, in some of the probiotic isolates, the production of the Vitamin B group was detected by thin layer chromatography. Breastmilk samples were collected from ۳۴ healthy women with an average age of ۲۷.۵ ± ۶.۵ years. Eighty-one colonies were isolated under microaerophilic conditions based on different morphological characteristics. Fungi, gram-positive and gram-negative bacteria were composed ۳۶%, ۳۴%, and ۳۰% of the isolates respectively. Two isolates with strong probiotic potential were molecularly examined and named Enterococcus faecalis (SUBC ۱۵۶), and E. faecalis (SUBC ۱۶۹). The highest resistance to acidity (۱۸%) and bile salt (-۱) was displayed by SUBC۱۶۹, while SUBC۱۵۶ survived lysozyme treatment and had a hydrophobicity of ۵۶%. SUBC۱۵۳ and SUBC۱۵۶ produced vitamin B۶ in a milk-containing MRS medium. Breast milk is recommended because of its probiotic qualities. Identifying those strains may confirm the true beneficial effect of probiotics in infant nutrition.

کلیدواژه ها:

Breast milk ، Lactic Acid Bacteria ، Lactobacillus ، Probiotics