Synergistic Antimicrobial Potential of Pistacia atlantica Gum Essential Oil and Camellia sinensis Methanolic Extract Against Pseudomonas aeruginosa

Pseudomonas aeruginosa, a versatile pathogen causing a wide range of infections, faces escalating resistance to conventional antibiotics, necessitating exploration of alternative substances. Plant extracts and essential oils, known for potent antibacterial compounds, emerge as promising candidates to combat P. aeruginosa effectively. Essential oil from Pistacia atlantica gum was extracted using a Clevenger apparatus, and Camellia sinensis (green tea) extract was obtained through maceration. Gas chromatography/mass spectrometry (GC-MS) and spectrophotometry identified compounds in the essential oil and extract. Antibacterial properties were assessed using the microdilution technique, and synergistic effects of combined substances were evaluated through the modified checkerboard method, utilizing the Fractional Inhibitory Concentration (FIC) index. P. aeruginosa (ATCC۲۷۸۵۳) was the target bacteria. Alpha-pinene and polyphenols were identified as predominant compounds in P. atlantica gum essential oil and C. sinensis extract, respectively. Both compounds exhibited comparable Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values, at ۶۲۵ and ۱۲۵۰ μg/ml, respectively. The FIC index calculation for the combination of essential oil and extract demonstrated enhanced antibacterial activity against P. aeruginosa. Results affirm the inhibitory and bactericidal properties of P. atlantica gum essential oil and C. sinensis extract against P. aeruginosa. Concurrent use exhibited synergistic antibacterial effects. This study suggests that these natural compounds, especially when combined, could serve as potent agents in the battle against P. aeruginosa infections, offering a potential avenue for the development of alternative antimicrobial strategies.

کلیدواژه ها:

Pseudomonas aeruginosa ، Pistacia atlantica ، Camellia sinensis ، Minimum inhibitory concentration ، Minimum bacteriocidal concentration ، Fractional Inhibitory Concentration