MicroRNA-۱۴۲-۳p chemo-sensitizing breast cancer to docetaxel: apoptosis and cell cycle arrest induction, and migration suppression

Docetaxel (DTX) is widely utilized in breast cancer treatment. However, cancer cell resistance has limited its anti-tumor efficacy. Some molecules called microRNAs (miRNAs), acting like fine-tuned switches, can influence how breast cancer develops and spreads. We conducted a study to examine if augmenting breast cancer cells with a particular molecule, known as miRNA-۱۴۲-۳p, could improve the efficacy of a widely used treatment called DTX. The expression level of miR-۱۴۲-۳p was initially assessed in MDA-MB-۴۶۸ cells. The miRNA transfection was performed to conduct additional experiments. The impact of a combined treatment involving DTX and miRNA-۱۴۲-۳p on both cell migration (by wound healing assay) and apoptosis (using annexin V/Propidium iodide staining) was examined. Cell viability was determined through the MTT assay, and gene expression was quantified using quantitative real-time polymerase chain reaction. The combined application of DTX and miRNA-۱۴۲-۳p resulted in a significant decrease in the expression of factors promoting tumor growth, such as SOX۲, Octamer ۴, HMGA۲, Kruppel-like factor ۴, and Bach-۱. Additionally, the combination of miRNA-۱۴۲-۳p and DTX initiated apoptotic cell death. Moreover, the progression of breast cancer cells was impeded by inducing cell cycle arrest at the G۱ phase. This combination also efficiently restrained the migration and invasion of breast cancer cells. The DTX or miRNA-۱۴۲-۳p alone can suppress malignant behavior and progression of breast cancer cells, but their combination elicits a synergistic effect that further enhances breast cancer inhibition. In summary, miRNA-۱۴۲-۳p transfection can be administered in conjunction with DTX therapy to enhance its cytotoxicity against breast cancer cells and prevent chemoresistance.

کلیدواژه ها:

Breast cancer ، Cancer therapy ، chemotherapy ، Docetaxel ، microRNA-۱۴۲-۳p