Biological Application of Dispersive Magnetic Solid Phase Extraction Using Fe۳O۴@Cuo&GO Nanocomposite and Ion Mobility Spectrometry: Determination of Aspirin

In this study, a dispersive magnetic solid phase extraction (DMSPE) was described by combining graphene oxide (GO) with Fe۳O۴ and CuO (Fe۳O۴@CuO&GO) for extraction and preconcentration of aspirin (ASP) in biological samples. The morphology and structure of the prepared nanocomposite was characterized and analyzed by XRD, SEM and FTIR techniques. Ion mobility spectrometry (IMS) method equipped to a corona discharge ionization source was exploited to determine ASA. The extraction parameters (desorption solvent, pH, adsorbent amount, extraction time and temperature) and also the operational parameters of IMS were investigated and optimized. Under the optimum conditions, the DMSPE–IMS method provided a linear range ۶.۰–۴۰.۰ ng for ASA with coefficient of determination R۲ = ۰.۹۹. The LOD and LOQ values were ۰.۹ and ۳.۰ ng for ASA, respectively. The repeatability of developed method was evaluated as relative standard deviation (RSD% = ۲.۷). The proposed method was also used to determine ASA in human plasma and serum as biological samples, which recovery results were within ۸۹.۰–۱۰۰.۰%.

کلیدواژه ها:

Nanocomposite ، Dispersive magnetic solid phase extraction ، Aspirin ، Ion Mobility Spectrometry ، Biological samples