Fabrication of Aligned Electrospun Polycaprolactone-Lignin Nanofibers for Peripheral Nervous Regeneration

Background: Damage to peripheral nervous tissue results inpain and significant disability due to disorder sensory and motorfunctions in the body. On the other hand, the structure andfunction complexity of the nervous system cause complicationsand challenges in peripheral nerve regeneration. Among severalmethods for treating nerve damage, tissue engineering hasemerged as a novel approach to the rapid treatment of damagednerve tissue. Electrospinning technique is an effective technologyto fabricate aligned fibrous membrane for stimulating theExtracellular Matrix (ECM) in the nervous system.Materials and Methods: In this study, electrospun polycaprolactone(PCL) and lignin fibers were fabricated and characterizedby SEM, contact angle and Fourier-transfer infrared(FTIR). Attachment and viability of PC۱۲ cells on PCL/ligninscaffold were evaluated by SEM and MTT assay.Results: The morphology and structure of fibers showed thatthe fabricated fibers were oriented, bead-free, and in nanoscalesize. The resulting data indicated that lignin can significantlyimprove the hydrophilicity properties of the scaffold. Attachmentand viability of PC۱۲ cells on PCL/lignin scaffold wereevaluated by SEM and MTT assay, which showed biocompatibilityof PCL/lignin scaffold.Conclusion: Our research indicates that electrospun PCL/lignin has good potential for nerve regeneration.

کلیدواژه ها:

Peripheral Nerve Regeneration ، Electrospinning ، Nanofiber Scaffold