Evaluation of ۵-fluorouracil related genes in breast cancer involved in the microtubule structure

Krüppel-like factors (KLFs) are implicated in several cellular functions. Numerousbiological processes, including cell stemness, proliferation, apoptosis, autophagy, and migration, havebeen linked to it. [۱]. KIF۲۰A is an essential protein for cell division and causes chromosome migrationduring mitosis. Therefore, it is necessary for the survival and morphogenesis of cells [۲]. The formationof microtubules is achieved by polymerizing the heterodimers of α- and β-tubulins. There are at leastnine isoforms of α-tubulin in humans, including tubulin α۸ (TUBA۸), and nine isoforms of β-tubulin inhumans including tubulin β۲A class IIA (TUBB۲A), β۲B class IIB (TUBB۲B) [۳]. One of the mostpopular chemotherapy agents for the treatment of solid tumors is ۵-fluorouracil (۵-FU). The currentstudy aimed to determine the effect of ۵-FU on tubulin gene expression [۴]. By utilizing NCBI’s GEOdataset and GEO۲R, the genes that are differently expressed under different experimental settings withinthe gene expression patterns caused by ۵-FU in breast cancer cell lines were identified, then theGSE۲۵۸۴ dataset was chosen. The results showed that in the treated cells, the expression of the TUBA۸,TUBB۲, KLF۵, and KIF۲۰A genes was reduced in comparison to control cells. Based on the results itcan be suggested that the ۵-FU may modulate the microtubule formation by downregulating the tubulingene expression.

کلیدواژه ها:

KLF۵ ، KIF۲۰A ، TUBB۲ ، TUBA۸ ، ۵-fluorouracil drug ، MCF۷ cells