Using the recommended protein marker gpd to investigate the phylogeny relationships among ۲۳ species of bioindicator fungal genus Lactarius

This research has been carried out in the form of examining the similarity of the gpd proteinamino acid sequence among the isolates of ۲۳ species of the bioindicator fungal genus Lactarius. Inthis study, ۴۸ amino acid sequences of gpd protein related to ۲۳ species of Lactarius fungi wereextracted from UniProt database and clustered by version ۱۱ of MEGA software. gpd is a highlyconserved enzyme in the glycolytic pathway and catalyzes the conversion of glyceraldehyde-۳-phosphate to glycerate-۱,۳-biphosphate. Lactarius is a genus of ectomycorrhizal fungi that has severaledible species. Species of this genus, commonly known as milk caps, are characterized by the milkyliquid (latex) they exude when cut or injured. These fungi act as environmental indicators and theirpresence in the environment is used to evaluate the quality of the environment. In this research, thementioned sequences were compared among ۴۸ isolates of this genus and showed similarities anddifferences, and the isolates of each species stood together and the species were clearly separated. Inthe end, it was observed that the gpd protein marker can distinguish the species of this genus well andis a suitable candidate for phylogenetic analyzes and taxonomic studies.

کلیدواژه ها:

gpd ، Lactarius ، Mycoindicator ، phylogenetic analysis ، phylogeny