Effect of eight weeks of high-intensity interval training and moderate-intensity continuous training with quercetin supplementation on the gene expression of FOXO۱ and ATG۵ in the liver of diabetic obese rats

Background: The use of complementary medicine in healthcare is increasing rapidly. Therefore, the aim of the current study was to investigate the effect of eight weeks of high-intensity interval training and moderate-intensity continuous training with quercetin supplementation on the gene expression of FOXO۱ and ATG۵ in the liver of diabetic obese rats. Methods: In this experimental study, ۴۲ male Wistar rats were considered research samples at eight weeks. Rats were induced with diabetes after eight weeks of a high-fat diet and familiarization with a laboratory environment and treadmill. Rats were divided into seven groups, and six rats were placed in each group (n=۶): healthy control group, diabetes control group, diabetic quercetin group, high-intensity interval training with diabetes group, moderate-intensity continuous training with diabetes group, diabetic high-intensity interval training with quercetin group, and diabetic moderate-intensity continuous training with quercetin group. For one training group, eight weeks of high-intensity interval training, and for the other group, eight weeks of moderate-intensity continuous training on the treadmill were performed. Seventy-two hours after the last training session, liver tissues were isolated to check the gene expression of FOXO۱ and ATG۵. One-way analysis of variance test was used to check the difference between groups by SPSS version ۲۶ software. Results: Findings showed that by inducing type ۲ diabetes, gene expression of FOXO۱ increased (۳.۱۴ unit) (P<۰.۰۰۱) and ATG۵ gene expression decreased (۰.۷۱ unit) (P<۰.۰۰۱). After eight weeks of training investigation, gene expression of FOXO۱ and ATG۵ decreased (P<۰.۰۰۱) and increased (P<۰.۰۰۱), respectively, in all training groups compared to the diabetic control group. There was no significant difference between the four training groups (P>۰/۰۵). Conclusion: Both exercises with and without quercetin had a modulating effect on the gene expression of indicators related to the process of autophagy and blood glucose levels in the liver of diabetic obese rats.

کلیدواژه ها:

High-intensity interval training ، Endurance Training ، Autophagy ، FOXO۱ Protein ، Human ، ATG۵ Protein ، Human ، Liver ، Diabetes Mellitus ، Type ۲